DETERMINATION OF ANTIRADICAL ACTIVITY OF Inula helenium L. PLANT FLOWER AND LEAF GROWING IN UZBEKISTAN

ABSTRACT

This study is aimed at the discovery of the antiradical activity of flower and leaf extracts of Inula helenium L. (Andiz) grown in Uzbekistan. Antiradical activity of plant extracts was performed with DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay. Then, different amounts (50, 100, 150, and 200 µL) of plant extracts were added to 3 mL solution of DPPH, and absorbance was measured at a wavelength of 517 nm using a spectrophotometer. The reduced absorbance was due to the scavenging of radicals in samples. The IC₅₀ was calculated from the plot, antiradical activity vs test concentration, concentration necessary to inhibit 50% of DPPH radicals. The Inula helenium L flower and leaf extracts had relatively good antiradical activity, IC₅₀ value 645.2 µL. Inula helenium L. may provide natural elements of antioxidant constituents favorable for human health or antioxidant compounds to protect oxidative stress diseases.  

АННОТАЦИЯ

В данном исследовании мы будем изучать антирадикальную активность экстрактов из цветов и листьев растения Inula helenium L. (девясил), выращенного в Узбекистане. Антирадикальная активность этих растительных экстрактов была определена с помощью метода поглощения свободных радикалов DPPH (2,2-дифенил-1-пикрилгидразил). Растительные экстракты (50, 100, 150 или 200 мкл) смешивали с 3 мл раствора DPPH, и оптическая плотность измерялась на спектрофотометре при 517 нм. Падение оптической плотности показывало способность образца гасить свободные радикалы. Значение IC₅₀ для фракции подавленных радикалов DPPH на основе ингибирования на 50% было проанализировано по графику концентраций в зависимости от антирадикальной активности. Inula helenium L. показывает мощную антирадикальную активность в ботанических экстрактах со значением IC₅₀ 645,2 мкл; Полученные данные указывают на то, что Inula helenium L. является жизнеспособным источником антиоксидантных соединений, которые полезны для человека и могут защищать здоровье при заболеваниях, характеризующихся окислительным стрессом.

Keywords: Inula helenium L., Andiz, antiradical activity, DPPH, antioxidant, plant extract.

Ключевые слова: Inula helenium L., девясил, антирадикальная активность, DPPH, антиоксидант, растительный экстракт.

INTRODUCTION

In fact, as much as 80% of the Earth's population uses traditional medicine for primary health care, including active consumption of youth extracts and parts [1; 194]. Only a few studies in previous years have explored the health benefits of consuming medicinal plants eaten in Part [2;32]. The Department of many medicinal plants has proved to be a good resource, as recent findings indicate. Natural antioxidants are abundant in phenolic compounds, as members comprise tocopherols, carotenoids, and ascorbic acid. Many years ago it was known that Andiz (Inula Helenium L.) flower and leaf is found strong in medicinal importance at play throughout the world with good nutritional value in the flower and leaf. Also known as Andiz (Inula Helenium L.) it was declared, for many years, that the flower and leaf of the Andiz has a great medicinal value globally, rich in nutritional values. As such, Inula Helenium L. flowers and leaves have been proposed for the treatment of peptic ulcer, gastric yawning, hyperlipidemia, heart vein disease, and cancer [3; 261-269]. An indication for this is based on Andiz (Inula Helenium L.) previous reports of its antioxidant properties such as base synapic acid esters and flavonoids in sprouts, which are explored in [4; 13-16]. In Uzbekistan, however, the (Inula Helenium L.) flower is abundant and there is no record about this flower's and leaf's antiradical activity. This work seeks to identify the chemical distribution of such a medicinal plant [5;5495]. It encompasses what are important points to keep in mind, being flowers, leaves, stalks, roots, or even taproots like the medicinal plants, the department of great ecology, and the economy. This information chain can give rise to multiple use output. Fibers, vitamins, and minerals (rich in fibers, vitamins, and minerals) present in flowers and leaves can be foods [6;3304]. Color has been associated with flowers of medicinal plants, pectin (for instance, which can provide a gel to create a candy flavor), and polyphenol antioxidants from Bandy. Most medicinal plants have food uses when feeding animals [7;897]. This inter-related structure of plant parts emphasizes not only their nutrients but their role as biodiversity. And in using them, we can build a more sustainable model of agriculture and health that keep both the soil and environment in balance and economic robustness [8;1215-1228].

METHODS

The antiradical activity of the extracts of Inula helenium L. was evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay. DPPH is a stable free radical which has been applied broadly to assess the antioxidant potential of plant extracts. A 0.812 mM solution of DPPH was prepared in ethanol using a 100 mL volumetric flask. The solution was shielded to keep it from light using aluminum foil, and stored in a dark region at room temperature for 30 min. In the control sample, 3 mL DPPH solution and 200 µL ethanol were added to a quartz cuvette and the absorbance (D1) was measured at 517 nm using a YOKE K7000 spectrophotometer. To experimental samples, different volumes of plant extract (50, 100, 150 and 200 µL) were mixed with 3 mL of DPPH solution. Ethanol was added to ensure a final volume of 3.2 mL in the cuvette [9;1133-1133, 10; 318-328]. The absorbance (D2) was examined at 517 nm for 10 min. The AA% was determined for the antiradical activity calculated from the formula:

                                     (1)

RESULTS

The antiradical activity of Inula helenium L. flower and leaf extracts was evaluated through the DPPH radical scavenging method. The decrease in absorbance of the DPPH solution indicated the radical scavenging ability of the plant extracts.

Figure 1. Antiradical activity of Inula helenium L. extracts

Figure 1 shows the change in absorbance of the DPPH solution after the addition of different volumes of plant extracts. As the concentration of the extract increased, the absorbance decreased, indicating stronger antiradical activity. The results of the flower and leaf extracts show the significant antioxidant activity.

DISCUSSION

Antiradical activity of plant extracts was analyzed further by plotting the percentage antiradical activity (AA%) versus the sample volume. The IC₅₀, defined as the concentration needed to inhibit 50% of the DPPH radicals, was determined through linear regression with the trend line. Sample volume and antiradical activity correlation is shown in figure 2. The IC₅₀ was calculated by using the equation of the trend line (y = mx + b). From them, we calculated the IC₅₀ value for flower and leaf extract of Inula helenium L. to be 645.2 µL (indicates Inula helenium L moderate antiradical activity). The observed antioxidant activity can be explained by the presence of phenolic compounds and flavonoids in plant extracts.

Figure 2. Graphical determination of IC₅₀ value of Inula helenium L.

Plant Flower and Leaf Growing in Uzbekistan on the mesh of AA% s and volumes determined in the 10th minute of the sample. The trend line transferred to the graph was calculated from the function (2) formula y=mx+b to 50% AA% manifesting volume (IC50) X=(y-b)/M: In place of the conclusion, it can be said that sample 1 exhibits antiradicality activity, in particular, its IC50 value was found to be 645.2 mcl.

                                  (2)

 CONCLUSION

Drawing on the results of this investigation, we have confirmed their antiradical activity, as evidenced by their DPPH assay based on flower and leaf extracts of Inula helenium L. growing in Uzbekistan. The IC₅₀ value was 645.2 µL, supporting the ability of the plant extracts to scavenge free radicals. The antioxidant efficiency for this plant might be related to phenolic compounds, flavonoids, and other bioactive substances. Inula helenium L. has thus far been proposed from this information as possibly a natural source of antioxidants, which may find the way into use as the basis of functional foods or compounds in pharmaceuticals. There should be a more precise identification and isolation of the relevant bioactive compounds responsible for the activity observed in antiradical studies.

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