Doctoral student, Department of Biotechnology, Tashkent Institute of Chemical Technology, Republic of Uzbekistan, Tashkent
GROWTH DYNAMICS OF Fusarium proliferatum IN DIFFERENT NUTRIENT MEDIA ISOLATED FROM GRAPEVINE
ABSTRACT
The article studied the release of mycotoxin and the growth dynamics of the fungus Fusarium proliferatum on various nutrient media, isolated from the varieties of Rizamat ota and Husaini from farm "Alijon Kuvonchbek bogi" Syrdarya region.
АННОТАЦИЯ
В статье изучена выделения микотоксина и динамика роста гриба Fusarium proliferatum на различных питательных средах, выделенного из сортов Ризамат ота и Хусайни фермерского хозяйства «Алижон Кувончбек боги» Сырдарьинской области.
Keywords: Fusarium proliferatum, mycotoxin, Chapeka Dox, Sabouraud Agar, Mendels, Potato Dextrose Agar, growth dynamics.
Ключевые слова: Fusarium proliferatum, микотоксин, Чапека Докса, Сабуро, Мендельс, КГА динамика роста.
Introduction. Grapes are an important plant in the agriculture of Uzbekistan. In 2021 the country's farms grow grapes on 90,000 hectares, 900,000 people are employed in permanent and seasonal employment, 52,000 hectares of new vineyards have been created over the past four years and 210 billion soums of subsidies have been allocated, and the share of grapes has doubled. To this end, we reviewed the extent to which Fusarium proliferatum has been studied in different countries based on the literature. Fusarium proliferatum causes Fusarium wilt or root rot in many plants. But the fungus has the ability to produce various mycotoxins. That is why scientists around the world are studying this mushroom on a large scale.
In particular, Pakistani scientists studied root rot in 120 specimens of grapes, 30 of which showed a typical appearance of the fungus Fusarium proliferatum (Fig. 1) [20, p.85-88]. When the potato-dextrose agar (KDA) is grown at a temperature of 25ºC, it initially turns white, cotton-colored (Fig. 2), purple and dark purple with increasing duration of cultivation, their macroconidia are thin, thin-walled cell wall 3 divided into up to 5 fragments, the size of the curved apical cell ranges from 20.9 to 45.2 × 3.2-7.1 μm, the microconidia are thin-layered, resembling aseptic particles and have a textured appearance, from 4.5 to 11.2 × Found to be between 2.3–4.1 μm (Fig. 3) [20, p.85-88].
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Figure 1. White-purple fungus growing on the top of the vine [Salman et al. 2018] |
Figure 2. Fusarium proliferatum colony on potato-dextrose agar |
Figure 3. Microscopic view of Fusarium proliferatum. ×400 |
We also studied the production of mycotoxins by the fungus Fusarium proliferatum on the basis of literature data. It has now been established that the fungus produces various mycotoxins. These mycotoxins have a major impact on food safety worldwide. Previously, Fusarium proliferatum was confused with morphologically related species. Now it is easy to separate using morphological and DNA analysis. It is widely distributed and has many hosts worldwide, releasing the mycotoxins Bauervoin, Fumonisin, Fusaproliferin, Fusaric acid, Fusarin, and Moniliformin [19, p.556]. Stuart P. Falk et al. studied the antimicrobial effects of Fusarium proliferatum G6 microconidia suspensions [22, p.1010-1017]. Israeli scientists isolated the cold-resistant G6 strain of mycoparasite Fusarium proliferatum using ultraviolet light. Fusarium species Plasmopara viticola, unsuitable for growth, showed 2-3 times faster growth than the parent strain when grown under easy growth conditions, i.e. at 13° C., when the isolated strain (designated as 1505) was grown. This rapid growth harvested (improved) the biological control of P.viticola found in leaf analysis [23, p.1062-1068]. From this it can be concluded that the mycotoxin produced by Fusarium can easily control P.viticola.
In 2012, Slovak scientists Zuzana Mašková et al. studied the type. In this case, F. proliferatum ranked high among the 11 most toxic species. Toxicity of these fungi was determined using chromatography (tonkosloynoy chromatografii). Tests have shown that F. proliferatum produces 100% of diacetoxistirpenol, NT-2 and T-2 toxins, 73% of NT-2 toxin and 50% of zearalenone [29, p.256-258].
The fungus produces different toxins according to the nutrient environment. Among the Slovak grapes, toxins of the genus Fusarium, among other fungi, F.proliferatum Chapeka yeast autolysate agar and sucrose and yeast extract agar (na agare s avtolizatom drojjey Chapeka i agare s drojjevym 3 to 400 kg of borax extract from 400 kg of sucrose) , 49850 to 259,500 μg / kg. The presence of boveritsin 2.24 mcg / kg, fumonizine B1 and fumonizine B2 in dried fruit from 500 to 2040 mcg / kg was noted [14, p.97-102.]. Polish scientists studied the formation of fumonizine (FB1 and FB2), moniliformine (MON) and ergosterol (ERG) in the fungi Fusarium oxysporum and Fusarium proliferatum, as well as the effect of temperature on the formation of mycotoxins. Fusarium proliferatum at 18 ° C was 720.0–1976.6 μg / g for FB1, 74.2–670.8 μg / g for FB2, and F. oxysporum had very low FB1 - 0.02–4 in 3 of 4 strains. 77 μg / g and FB2 0.02–2.15 μg / g of fumonizine were formed [26, p.608-615].
According to, in China [27], [28], [7], [25, P.2306-2313], [2, p.1528], in Brazil [11], in Italy [5, р.37-46], in Russia [12], [30, с.160], in India [17], [13, р.704], in USA [15, p.1009], [18, р.2102-2110], [3, р.550], [24, р.1526], [8, р.1931-1939], [21, р.909], [9, р.843], in Argentina [6, р.1405], [19], in Oman [1, р.284], in Tunisia [10, р.1217], in Switzerland [4, p.1010-1017] was studied microbiological, cultural, biochemical, molecular genetic properties of the fungus.
Savchuk N.V. in her dissertation [30, с.160] identified F.proliferatum, belonging to the genus Fusarium, causing the formation of Fusarium desiccation in grape plants, and developed an environmentally complex method of control through a comprehensive assessment of damage.
Falk S.P. and others in 1998 [4, p.1010-1017] studied the effect of F.proliferatum against Plasmopara viticola. According to the results of the experiment, the process of sporulation in grape leaves was stopped by 97%.
Materials and Methods. In April 2021, we decided to study the growth dynamics of the Fus.proliferatum fungus isolated from the Rizamat ota and Husaini varieties by the “Alijon Kuvonchbek Bogi” farm on various nutrient media. We chose Chapeka Dox, Saburo, Mendels and KGA as culture media. F.proliferatum was sown on selected nutrient media and placed in a Memmert IPP 500 thermostat at 30ºC.The diameter of the colony formed on the nutrient media of the fungus was measured from 3 to 10 days.
Results. The fungus grown on different nutrient media gave different morphological and physiological results. Table 1 shows the growth dynamics. We know that the composition of the nutrient medium affects not only the physiological state of microorganisms, but also their morphological appearance. In the studied Mendels nutrient medium, the fungus formed a reddish-white colony, unlike in other mediums (Figure 1).
Table 1.
Growth dynamics of Fusarium proliferatum in different nutrient media
Potato Dextrose Agar, mm. |
Sabouraud Agar, mm. |
Mendels, mm |
Chapeka Dox, mm |
91 |
60 |
69 |
92 |
Potato Dextrose |
Mendels |
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Sabouraud Agar |
Chapeka Dox |
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Figure 4. Colony formation of different sizes by Fusarium proliferatum in different nutrient media
Conclusion. According to the results, on the 8th day, F.proliferatum formed the largest colonies on nutrient media in Chapeka Dox (92 mm) and Potato Dextrose Agar (91 mm). Next was Mendels (69mm), the smallest diameter Sabouraud Agar (60mm) found in culture medium. In fact, growth was normal on all 4 media. However, Chapeka Dox and KGA proved to be the optimal medium for Fusarium proliferatum. For further research, it is planned to conduct experiments on the basis of the Chapeka Dox nutrient medium to determine the effectiveness of biopreparations against the phytopathogenic fungus Fusarium.
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